A Novel Approach for Obtaining and Identifying Monoclonal Antibodies That React with Differentiation-specific Antigens Using Human Hybrid Cells1
نویسندگان
چکیده
HeLa x keratinocyte hybrid cells have been used as immunogens to generate monoclonal antibodies against differentiation-specific antigens. A key feature of the experimental system is that the hybrid cells are undifferentiated in culture and yet terminally differentiate in the mouse. The properties of these cells allow one a facile approach to obtaining and characterizing monoclonal antibodies against differentiation-specific an tigens. Use of hybrid cells derived from HeLa x differentiated cell fusions as immunogens should prove to be a general method for identifying differentiation-specific antigens from a variety of differentiated cell types.
منابع مشابه
A novel approach for obtaining and identifying monoclonal antibodies that react with differentiation-specific antigens using human hybrid cells.
HeLa X keratinocyte hybrid cells have been used as immunogens to generate monoclonal antibodies against differentiation-specific antigens. A key feature of the experimental system is that the hybrid cells are undifferentiated in culture and yet terminally differentiate in the mouse. The properties of these cells allow one a facile approach to obtaining and characterizing monoclonal antibodies a...
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